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Expedeon/ELISA-ONE™ for STAT3/ELISA-ONE for total STAT3, 1 x 96 Well Plate Kit/ELK014
产品编号:ELK014
市  场 价:¥7500.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$375.00
品      牌: Expedeon
公      司:Expedeon
公司分类:
Expedeon/ELISA-ONE™ for STAT3/ELISA-ONE for total STAT3, 1 x 96 Well Plate Kit/ELK014
商品介绍

ELISA-ONE for Phospho STAT3 (pY705)

Specificity

ELISA-ONE for phospho STAT3 (pY705) are ready to use ELISA kits for the detection of endogenous levels of STAT3 when phosphorylated at Tyr705.

ELISA-ONE for phospho STAT3 (pY705) is comprised of a precoated 96 well (12×8 strips) plate and an antibody pair, recognizing the phospho-Tyr705 epitope and a distal epitope of STAT3.

The antibody pair recognizes human STAT3. However, other species should be tested on a case by case basis.

ELISA-ONE for Total STAT3

Specificity

ELISA-ONE for total STAT3 are ready to use ELISA kits for the detection of endogenous levels of STAT3 irrespective of phosphorylation status.

ELISA-ONE for total STAT3 is comprised of a precoated 96 well (12×8 strips) plate and an antibody pair, recognizing two invariants epitopes of STAT3.

The antibody pair recognizes human STAT3. However, other species should be tested on a case by case basis.

ELISA-ONE for Phospho & Total STAT3

Specificity

ELISA-ONE for phospho and total STAT3 are ready to use ELISA kits for the detection of endogenous levels of STAT3 in its phosphorylated and unphosphorylated form.

ELISA-ONE for phospho and total STAT3 is comprised of a precoated 96 well (12×8 strips) plate and two antibody pairs, recognizing the phospho-Tyr705 epitope and an invariant epitope of STAT3.

The antibody pair recognizes human STAT3. However, other species should be tested on a case by case basis.

Technical Specifications

GenBank Accessions
STAT3α NP_644805.1
STAT3β NP_998827.1

Species Cross-reactivity

Tested: Human

Kit Contents

  • 1x 96 well (12×8 strips) CaptSure assay plate
  • 1x adherent plate seal
  • 1x Lysis Buffer (5X)
  • 1x Enhancer Solution
  • 1x Wash Buffer (10X)
  • 1x Detection Reagent
  • 1x Stop Solution
  • 1x Lyophilized Positive Control Lysate
  • 1x Capture Antibody Reagent 1
  • 1x Capture Antibody Reagent 2 (phospho / total target kits only)
  • 1x Detection Antibody Reagent 1
  • 1x Detection Antibody Reagent 2 (phospho / total target kits only).

STAT3 Signaling Pathway

STAT3 phosphorylation was first described as an IL‐6 mediated response but has since been found to be induced by many stimuli. STAT3 exists as two splice variants (STAT3α and STAT3β), the expression of which appear cell type and cell maturation dependent. STAT3 is phosphorylated at Tyr705 by activated JAK proteins, or by various receptor tyrosine kinases, which induces dimerization and translocation to the nucleus. STAT3α (but not STAT3β) is also phosphorylated at Ser727 in a MAPK and mTOR dependent mechanism, which enhances transcriptional activity.

STAT3 has been linked to several processes important for tumor progression and has been found to be constitutively active in certain tumor cells. Prooncogenic activities attributed to STAT3 include the upregulation of transcription of angiogenic proteins, and immune suppressive proteins.

DIAGRAM_ELISA-ONE_STAT3 pathway

STAT3 References

Guanizo, AC., Fernando, CD., Garama, DJ., Gough, DJ. STAT3: a multifaceted oncoprotein (Review). Growth Factors. 2018 Apr;36(1-2):1–14. doi: 10.1080/08977194.2018.1473393. Epub 2018 Jun 6.

Johnson, DE., O’Keefe, RA., Grandis, JR. Targeting the IL-6/JAK/STAT3signalling axis in cancer (Review). Nat Rev Clin Oncol. 2018 Apr;15(4):234–248. doi: 10.1038/nrclinonc.2018.8. Epub 2018 Feb 6.

Park, HS., Quan, KT., Han, JH., Jung, SH., Lee, DH., Jo, E., Lim, TW., Heo, KS., Na, M., Myung, CS. Rubiarbonone C inhibits platelet-derived growth factor-induced proliferation and migration of vascular smooth muscle cells through the focal adhesion kinase, MAPK and STAT3 Tyr705 signalling pathways. Br J Pharmacol. 2017 Nov;174(22):4140–4154. doi: 10.1111/bph.13986. Epub 2017 Sep 22.

Wingelhofer, B., Neubauer, HA., Valent, P., Han, X., Constantinescu, SN., Gunning, PT., Müller, M., Moriggl, R. Implications ofSTAT3 and STAT5 signaling on gene regulation and chromatin remodeling in hematopoietic cancer (Review). Leukemia. 2018 Aug;32(8):1713–1726. doi: 10.1038/s41375-018-0117-x. Epub 2018 Mar 27.

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